Open AccessWbjA Adds Glucose To Complete the O-Antigen Trisaccharide Repeating Unit of the Lipopolysaccharide of Pseudomonas aeruginosa Serogroup O11
Author(s) -
Charles R. Dean,
Anup Datta,
Russell W. Carlson,
Joanna B. Goldberg
Publication year - 2002
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.184.1.323-326.2002
Subject(s) - biology , pseudomonas aeruginosa , microbiology and biotechnology , trisaccharide , lipopolysaccharide , mutant , antigen , pseudomonadales , pseudomonadaceae , glycosyltransferase , bacteria , lipid a , strain (injury) , biochemistry , immunology , enzyme , gene , genetics , anatomy
Lipopolysaccharide from a wbjA mutant, deficient in a putative glycosyltransferase from Pseudomonas aeruginosa serogroup O11, was compared to that from an O-antigen polymerase mutant. Results suggest that WbjA adds the terminal glucose to complete the serogroup O11 O-antigen unit and identifies the biological repeating unit as [-2)-beta-D-glucose-(1-3)-alpha-L-N-acetylfucosamine-(1-3)-beta-D-N-acetylfucosamine-(1].