Open AccessCharacterization of Intragenic Recombination in a Hyperthermophilic Archaeon via Conjugational DNA Exchange
Author(s) -
Michelle S. Reilly,
Dennis W. Grogan
Publication year - 2001
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.183.9.2943-2946.2001
Subject(s) - sulfolobus acidocaldarius , biology , genetics , frameshift mutation , sulfolobus , mutation , mutant , recombination , dna , gene , euryarchaeota , microbiology and biotechnology , archaea
Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontaneous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed orotidine-5'-monophosphate decarboxylase activity (encoded by pyrF), whereas base pair substitutions and an 18-bp deletion had no effect.