Open AccessPhase-Variable Expression of an Operon Encoding Extracellular Alkaline Protease, a Serine Protease Homolog, and Lipase in Pseudomonas brassicacearum
Author(s) -
Philippe Chabeaud,
Arjan de Groot,
Wilbert Bitter,
Jan Tommassen,
Thierry Heulin,
Wafa Achouak
Publication year - 2001
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.183.6.2117-2120.2001
Subject(s) - operon , biology , tmprss6 , serine protease , serine , protease , lipase , lac operon , masp1 , extracellular , biochemistry , pseudomonas , gene , microbiology and biotechnology , enzyme , genetics , gene expression , mutant , bacteria
The rhizobacterium Pseudomonas brassicacearum forms phenotypic variants which do not show extracellular protease and lipase activity. The operon encoding these enzymes, a serine protease homolog, and a type I secretion machinery was characterized. Transcriptional lacZ gene fusions revealed that the expression of the operon is under the control of phase variation.