Open AccessTFE, an Archaeal Transcription Factor in Methanobacterium thermoautotrophicum Related to Eucaryal Transcription Factor TFIIEα
Author(s) -
Brian L. Hanzelka,
Trevor J. Darcy,
John N. Reeve
Publication year - 2001
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.183.5.1813-1818.2001
Subject(s) - transcription factor ii a , biology , transcription factor ii e , general transcription factor , transcription factor ii d , transcription factor ii b , transcription factor ii f , taf2 , promoter , transcription factor , microbiology and biotechnology , transcription preinitiation complex , transcription (linguistics) , activator (genetics) , rna polymerase ii holoenzyme , rna polymerase ii , sigma factor , gene , genetics , gene expression , linguistics , philosophy
In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the alpha-subunits of eucaryal general transcription factor TFIIE. The recombinant MTH1669 gene product has been purified and shown to stimulate transcription in vitro from M. thermoautotrophicum promoters that were almost inactive or much less active in reaction mixtures that contained only M. thermoautotrophicum RNA polymerase, TATA-binding protein and transcription factor B. As all complete archaeal genome sequences contain an MTH1669 homolog, the protein encoded by this gene is apparently the first characterized example of a transcription activator, here designated TFE, that may be universally present in the Archaea.