Open AccessDistribution of the Ssu DAT1I Restriction-Modification System among Different Serotypes of Streptococcus suis
Author(s) -
Tsutomu Sekizaki,
Makoto Osaki,
Daisuke Takamatsu,
Yoshihiro Shimoji
Publication year - 2001
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.183.18.5436-5440.2001
Subject(s) - biology , serotype , restriction enzyme , isoschizomer , genetics , locus (genetics) , microbiology and biotechnology , gene , virology , gene expression , dna methylation
The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a field isolate of serotype 1/2 and the reference strains of serotypes 3, 7, 23, and 26 among 29 strains of different serotypes examined in this study. The R-M gene sequences in serotypes 1/2, 3, 7, and 23 were very similar to those of SsuDAT1I, whereas those in serotype 26 were less similar. These results indicate intraspecies recombination among them and genetic divergence through their evolution.