Open AccessCorrection of the DNA Sequence of the regB Gene of Rhodobacter capsulatus with Implications for the Membrane Topology of the Sensor Kinase RegB
Author(s) -
Weizhen Chen,
Andreas Jäger,
Gabriele Klug
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.3.818-820.2000
Subject(s) - rhodobacter , biology , histidine kinase , transmembrane domain , transmembrane protein , gene , histidine , peptide sequence , membrane topology , computational biology , topology (electrical circuits) , biochemistry , amino acid , receptor , mutant , mathematics , combinatorics
We corrected the previously published sequence for theregB gene, which encodes a histidine sensor kinase inRhodobacter capsulatus . The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.