Open AccessHigh-Frequency Flp Recombinase-Mediated Inversions of the oriC -Containing Region of the Pseudomonas aeruginosa Genome
Author(s) -
Nazir Barekzi,
Kerry Beinlich,
Tung T. Hoang,
Xuan-Quynh T. Pham,
RoxAnn R. Karkhoff-Schweizer,
Herbert P. Schweizer
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.24.7070-7074.2000
Subject(s) - biology , recombinase , chromosomal inversion , pseudomonas aeruginosa , genetics , operon , chromosome , genome , recombination , circular bacterial chromosome , gene , bacteria , escherichia coli , karyotype
The genomes of the two clonally derivedPseudomonas aeruginosa prototypic strains PAO1 and DSM-1707 differ by the presence of a 2.19-Mb inversion includingoriC . Integration of two Flp recombinase target sites near therrn operons containing the inversion endpoints in PAO1 led to Flp-catalyzed inversion of the intervening 1.59-Mb fragment, includingoriC , at high frequencies (83%), favoring the chromosome configuration found in DSM-1707. The results indicate that theoriC -containing region of theP. aeruginosa chromosome can readily undergo and tolerate large inversions.