Open AccessGenetic Analysis of Peptidoglycan Biosynthesis in Mycobacteria: Characterization of a ddlA Mutant of Mycobacterium smegmatis
Author(s) -
Aimee E. Belanger,
Joelle C. Porter,
Graham F. Hatfull
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.23.6854-6856.2000
Subject(s) - mycobacterium smegmatis , biology , mutant , dna ligase , peptidoglycan , mutation , mycobacterium , alanine , biochemistry , enzyme , recombinant dna , biosynthesis , gene , microbiology and biotechnology , mycobacterium tuberculosis , genetics , amino acid , bacteria , medicine , tuberculosis , pathology
A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding D-alanine:D-alanine ligase. Enzymatic assays using recombinant proteins and D-cycloserine susceptibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo.