Open AccessThe Amino Terminus of Salmonella enterica Serovar Typhimurium ς 54 Is Required for Interactions with an Enhancer-Binding Protein and Binding to Fork Junction DNA
Author(s) -
Mary T. Kelly,
Timothy R. Hoover
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.2.513-517.2000
Subject(s) - biology , enhancer , salmonella enterica , transcription (linguistics) , dna , dna binding protein , microbiology and biotechnology , binding site , polymerase , transcription factor , binding protein , biochemistry , gene , escherichia coli , linguistics , philosophy
Transcription initiation by the ς54 -RNA polymerase holoenzyme requires an enhancer-binding protein that is thought to contact ς54 to activate transcription. To identify potential enhancer-binding protein contact sites in ς54 , we compared the abilities of wild-type and truncated forms ofSalmonella enterica serovar Typhimurium ς54 to interact with the enhancer-binding protein DctD in a chemical cross-linking assay. Removal of two regions in the amino-terminal portion of ς54 , residues 57 to 105 and residues 144 to 179, prevented cross-linking, but removal of either region alone did not. In addition, deletion of 56 amino-terminal residues of ς54 (region I) reduced the affinity of the protein for a fork junction DNA probe.