Open AccessPositioning of Region 4 of the Escherichia coli RNA Polymerase ς 70 Subunit by a Transcription Activator
Author(s) -
Jonathan A. Bown,
Annie Kolb,
Claude F. Meares,
Akira Ishihama,
Stephen Minchin,
Stephen J. W. Busby
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.10.2982-2984.2000
Subject(s) - biology , rna polymerase , camp receptor protein , microbiology and biotechnology , transcription (linguistics) , protein subunit , escherichia coli , polymerase , activator (genetics) , dna , biochemistry , promoter , receptor , gene , gene expression , linguistics , philosophy
A DNA cleavage reagent, specifically tethered to residue 581 of theEscherichia coli RNA polymerase ς70 subunit, has been used to investigate the location of ς70 region 4 in different complexes at thegalp 1 promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at thegalp 1 promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of ς70 region 4.