Open AccessInhibition of RecA-mediated cleavage in covalent dimers of UmuD
Author(s) -
M H Lee,
Angelina Guzzo,
Graham C. Walker
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.24.7304-7307.1996
Subject(s) - cleavage (geology) , dimer , covalent bond , monomer , biology , incubation , bond cleavage , disulfide bond , stereochemistry , biochemistry , biophysics , chemistry , organic chemistry , catalysis , paleontology , fracture (geology) , polymer
Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.