Open AccessPositions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site
Author(s) -
Carol Peña,
Judy Stoner,
Graham F. Hatfull
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.18.5533-5536.1996
Subject(s) - biology , mycobacterium smegmatis , genetics , dna , integrases , gene , bacteriophage , recombination , microbiology and biotechnology , escherichia coli , mycobacterium tuberculosis , medicine , tuberculosis , pathology
Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.