Open AccessEscherichia coli DNA repair genes radA and sms are the same gene
Author(s) -
Yifu Song,
Neil J. Sargentini
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.16.5045-5048.1996
Subject(s) - biology , complementation , mutant , escherichia coli , gene , plasmid , genetics , ethyl methanesulfonate , microbiology and biotechnology , mutation
Escherichia coli strains carrying radA100 or sms mutations were identical in their sensitivities to either methyl methanesulfonate or UV radiation treatment and in their plasmid complementation patterns for UV radiation survival. DNA sequencing analysis of the radA mutant and radA+ strains and comparison of their sequences with the published sms gene sequence showed the radA mutant to differ only by a G-to-A transition mutation, which is predicted to change a cysteine in a zinc-finger motif to tyrosine. The sms gene is concluded to be identical to the previously described radA gene.