Open AccessSingle-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli
Author(s) -
William A. Rosche,
Adam Jaworski,
Seongman Kang,
Shan F. Kramer,
Jacquelynn E. Larson,
David P. Geidroc,
Robert D. Wells,
Richard R. Sinden
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.16.5042-5044.1996
Subject(s) - escherichia coli , biology , dna , dna binding protein , mutation , genetics , microbiology and biotechnology , gene , transcription factor
The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded-DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.