Open AccessMembers of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae
Author(s) -
José L. López-Ribot,
W. LaJean Chaffin
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.15.4724-4726.1996
Subject(s) - saccharomyces cerevisiae , biology , cell wall , hsp70 , cell , biotinylation , cytosol , heat shock protein , cell membrane , extracellular , immunofluorescence , yeast , microbiology and biotechnology , mutant , western blot , biochemistry , gene , genetics , antibody , enzyme
Western blot (immunoblot) analysis of cell wall and cytosolic extracts obtained from parental and ssa1 and ssa2 single- and double-mutant strains of Saccharomyces cerevisiae showed that the heat shock protein 70 (Hsp70) products of these genes, previously thought to be restricted to the cell interior, are also present in the cell wall. A cell wall location was further confirmed by indirect immunofluorescence with intact cells and biotinylation of extracellular Hsp70. Hsp70s have been implicated in translocation across the membrane and as molecular chaperones, and changes in the profile of cell wall proteins suggested that these proteins may have a similar role in the cell wall.