Open AccessCloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage
Author(s) -
Malgorzata M. Slupska,
Claudia Baikalov,
Wendy M. Luther,
JuHuei Chiang,
Yu-Ling Wei,
Jeffrey H Miller
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.13.3885-3892.1996
Subject(s) - biology , gene , genetics , escherichia coli , exon , cloning (programming) , complementary dna , genomic dna , intron , dna , microbiology and biotechnology , dna damage , dna repair , computer science , programming language
We have cloned the human mutY gene (hMYH) from both genomic and cDNA libraries. The human gene contains 15 introns and is 7.1 kb long. The 16 exons encode a protein of 535 amino acids that displays 41% identity to the Escherichia coli protein, which provides an important function in the repair of oxidative damage to DNA and helps to prevent mutations from oxidative lesions. The human mutY gene maps on the short arm of chromosome 1, between p32.1 and p34.3.