Open AccessFrur mediates catabolite activation of pyruvate kinase (pykF) gene expression in Escherichia coli
Author(s) -
Stefan A. Bledig,
Tom M. Ramseier,
Milton H. Saier
Publication year - 1996
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.178.1.280-283.1996
Subject(s) - catabolite repression , biology , escherichia coli , lac operon , pyruvate kinase , glycolysis , gene expression , gene , biochemistry , regulation of gene expression , enterobacteriaceae , enzyme , mutant
Expression of a pykF-lacZ fusion was studied as a function of the carbon source in wild-type strains and strains lacking or overproducing the FruR protein of Escherichia coli. FruR controls the response to the carbon source by repressing pykF expression more strongly under gluconeogenic than under glycolytic conditions, a phenomenon we term catabolite activation.