Open AccessProtein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs
Author(s) -
Manuel García,
Cyril Lutze-Wallace,
A.S. Denes,
M.D. Eaglesome,
Elisabet Holst,
Martin J. Blaser
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.8.1976-1980.1995
Subject(s) - biology , campylobacter fetus , s layer , antigenic variation , antigen , fetus , monoclonal antibody , epitope , campylobacter , microbiology and biotechnology , genomic dna , virology , antibody , genetics , dna , gene , bacteria , pregnancy
Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement.