Open AccessMutations in GltC that increase Bacillus subtilis gltA expression
Author(s) -
Boris R. Belitsky,
Abraham L. Sonenshein
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.19.5696-5700.1995
Subject(s) - biology , bacillus subtilis , mutant , gene , wild type , gene expression , promoter , sigma factor , genetics , transcription (linguistics) , mutation , microbiology and biotechnology , bacteria , linguistics , philosophy
Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA became much less dependent on a nitrogen source-associated signal.