Open AccessOccurrence of the regulatory nucleotides ppGpp and pppGpp following induction of the stringent response in staphylococci
Author(s) -
Robert Cassels,
B Oliva,
Donald P. Knowles
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.17.5161-5165.1995
Subject(s) - stringent response , biology , nucleotide , escherichia coli , microbiology and biotechnology , sos response , mupirocin , biochemistry , staphylococcus aureus , bacteria , genetics , gene , methicillin resistant staphylococcus aureus
The stringent response in Escherichia coli and many other organisms is regulated by the nucleotides ppGpp and pppGpp. We show here for the first time that at least six staphylococcal species also synthesize ppGpp and pppGpp upon induction of the stringent response by mupirocin. Spots corresponding to ppGpp and pppGpp on thin-layer chromatograms suggest that pppGpp is the principal regulatory nucleotide synthesized by staphylococci in response to mupirocin, rather than ppGpp as in E. coli.