Open AccessMutagenesis of the IS1 transposase: importance of a His-Arg-Tyr triad for activity
Author(s) -
Marie-Claude Serre,
Catherine Turlan,
MarieLine Bortolin,
Michaël Chandler
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.17.5070-5077.1995
Subject(s) - transposase , biology , integrases , mutant , genetics , mutation , mutagenesis , transposable element , biochemistry , microbiology and biotechnology , recombination , gene
Inspection of the primary sequence of the IS1 transposase suggested that it carries residues which are characteristic of the active site of integrases of the bacteriophage lambda family (Int). In particular, these include a highly conserved triad: His-Arg-Tyr. The properties of mutants made at each of these positions were investigated in vivo. The results of several different assays confirm that each is important for transposase activity. Moreover, as in the case of members of the Int family, different mutations of the His residue exhibited different effects. In a particular, His-to-Leu mutation resulted in complete inactivation whereas the equivalent His-to-Gln mutation retained low but significant levels of activity.