Open AccessPossible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis
Author(s) -
Smruti J. Vidwans,
Keith Ireton,
Andalan D. Grossman
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.11.3308-3311.1995
Subject(s) - bacillus subtilis , biology , lac operon , gtp' , mutation , phosphorylation , microbiology and biotechnology , lac repressor , gene , genetics , biochemistry , gene expression , enzyme , bacteria
We fused obg, encoding an essential GTP-binding protein in Bacillus subtilis, to the LacI-repressible, IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter Pspac. Depletion of Obg, following removal of IPTG, caused a defect in sporulation and in expression of sporulation genes that are activated by Spo0A approximately P. These defects were significantly relieved by a mutation in spo0A (rvtA11) that bypasses the normal phosphorylation pathway, indicating that Obg might normally be required, either directly or indirectly, to stimulate activity of the phosphorelay that activates Spo0A.