Open AccessThe role of ribonuclease H in multicopy single-stranded DNA synthesis in retron-Ec73 and retron-Ec107 of Escherichia coli
Author(s) -
Tadashi Shimamoto,
Mikio Shimada,
Masayori Inouye,
Sumiko Inouye
Publication year - 1995
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.177.1.264-267.1995
Subject(s) - biology , rnase h , rnase p , reverse transcriptase , ribonuclease , rnase mrp , dna , escherichia coli , microbiology and biotechnology , genetics , rna , gene
Bacterial reverse transcriptase is responsible for the synthesis of multicopy single-stranded DNA (msDNA). Reverse transcriptases from retron-Ec73 and retron-Ec107 do not contain an RNase H domain. Cellular RNase H is therefore considered to be required to make the mature form of msDNA. We found that RNase HI, but not RNase HII, is required for the production of the mature form of both msDNAs.