Open AccessLocations of genetic markers on the physical map of the chromosome of Neisseria gonorrhoeae FA1090
Author(s) -
J. A. F. Dempsey,
Janne G. Can
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.7.2055-2060.1994
Subject(s) - biology , neisseria gonorrhoeae , genetics , southern blot , chromosome , restriction map , gene mapping , restriction enzyme , genetic marker , gene , strain (injury) , restriction fragment length polymorphism , restriction fragment , hybridization probe , microbiology and biotechnology , polymerase chain reaction , base sequence , anatomy
To increase the utility of the previously constructed physical map of the chromosome of Neisseria gonorrhoeae FA1090, 28 additional genetic markers were localized on the map. Cloned gonococcal genes were used to probe Southern blots of restriction enzyme-digested DNA separated on pulsed-field gels, thus identifying the fragment in each of several digests to which the probe hybridized and the map location of each gene. The addition of the new markers brings the total number of mapped loci for this strain to 68; the locations of all of those markers on the updated map are shown.