Open AccessAbsence of a role for DNA polymerase II in SOS-induced translesion bypass of phi X174
Author(s) -
Yoke W. Kow,
Gustavo Faúndez,
Sharon Hays,
Cynthia A. Bonner,
Myron F. Goodman,
Susan S. Wallace
Publication year - 1993
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.175.2.561-564.1993
Subject(s) - biology , dna polymerase , pyrimidine dimer , dna polymerase ii , mutagenesis , ap site , dna clamp , dna polymerase i , polymerase , dna repair , microbiology and biotechnology , dna , dna replication , dna damage , biochemistry , mutation , polymerase chain reaction , gene , reverse transcriptase
In order to examine the possible role of Escherichia coli DNA polymerase II in SOS-induced translesion bypass, Weigle reactivation and mutation induction were measured with single-stranded phi X174 transfecting DNA containing individual lesions. No decrease in bypass of thymine glycol or cyclobutane pyrimidine dimers in the absence of DNA polymerase II was observed. Furthermore, DNA polymerase II did not affect bypass of abasic sites when either survival or mutagenesis was the endpoint. Lastly, repair of gapped DNA molecules, intermediates in methyl-directed mismatch repair, was also unaffected by the presence or absence of DNA polymerase II.