Open AccessReplication of the R6K plasmid during the Escherichia coli cell cycle
Author(s) -
Jay D. Keasling,
Bernhard Ø. Palsson,
Stephen Cooper
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.3.1060-1062.1992
Subject(s) - biology , plasmid , cell cycle , dna replication , cell division , escherichia coli , origin of replication , microbiology and biotechnology , plasmid preparation , dna , s phase , origin recognition complex , eukaryotic dna replication , cell , genetics , gene , pbr322
The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner.