Open AccessRole of PhoU in phosphate transport and alkaline phosphatase regulation
Author(s) -
Marco Muda,
Naraya. Rao,
Annamaria Torriani
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.24.8057-8064.1992
Subject(s) - biology , psychological repression , phosphatase , function (biology) , escherichia coli , mutation , genetics , alkaline phosphatase , biochemistry , microbiology and biotechnology , gene , enzyme , gene expression
The negative regulatory function of PhoU in alkaline phosphatase (AP) was suggested by the behavior of K10 phoU35 carrying a missense mutation whose product was detected by immunoblotting. To define more clearly the regulatory function of this protein for the synthesis of AP, we constructed a null mutation. The constitutive synthesis of AP in this phoU deletion strain confirmed the negative role of PhoU. However, the expression of the PhoU protein from an isopropyl-beta-D-thiogalactopyranoside-inducible promoter had no effect on the repression of AP synthesis. Furthermore, the involvement of PhoU in free-Pi uptake was demonstrated. These results provide evidence that PhoU participates in Pi transport and in the regulatory role of the phosphate-specific transport system.