Open AccessDoublet translocation at GGA is mediated directly by mutant tRNA(2Gly)
Author(s) -
Frances T. Pagel,
Thérèse M.F. Tuohy,
John F. Atkins,
Emanuel J. Murgola
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.12.4179-4182.1992
Subject(s) - transfer rna , frameshift mutation , biology , mutant , genetics , translation (biology) , mutation , suppressor , chromosomal translocation , translational frameshift , ribosome , rna , gene , messenger rna
Members of the sufS class of -1 frameshift suppressors have alterations of the GGA/G-decoding tRNA(2Gly). Suppressor-promoted frameshifting at GGA was shown in this study to be directly mediated by the mutant tRNA(2Gly). We disproved the possibility that, in the presence of the compromised mutant tRNA(2Gly), either wild-type tRNA(1Gly), wild-type tRNA(3Gly), a GGA-reading mutant form of tRNA(3Gly), or any other agent suppresses the frameshift mutation trpE91.