Open AccessSequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene
Author(s) -
Tao Tao,
Robert Blumenthal
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.10.3395-3398.1992
Subject(s) - open reading frame , biology , gene , genetics , regulator gene , endonuclease , regulator , reading (process) , sequence (biology) , regulation of gene expression , peptide sequence , political science , law
An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.