Open AccessA vector for analysis of promoters in the cyanobacterium Anabaena sp. strain PCC 7120
Author(s) -
Jean D. Lang,
Robert Haselkorn
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.8.2729-2731.1991
Subject(s) - biology , anabaena , chloramphenicol acetyltransferase , plasmid , multiple cloning site , promoter , escherichia coli , gene , shuttle vector , vector (molecular biology) , cloning (programming) , microbiology and biotechnology , transcription (linguistics) , genetics , recombinant dna , cyanobacteria , gene expression , bacteria , computer science , programming language , linguistics , philosophy
A plasmid vector containing a multiple-cloning site followed by a promoterless chloramphenicol acetyltransferase (cat) gene, protected by transcription terminators and mobilizable by conjugation from Escherichia coli into Anabaena sp. strain PCC 7120, was constructed. The utility of the vector was shown by deletion analysis of the promoter region of the Anabaena psbB gene.