Open AccessLocalization of outer surface proteins A and B in both the outer membrane and intracellular compartments of Borrelia burgdorferi
Author(s) -
John S. Brusca,
Alasdair W. McDowall,
Michael V. Norgard,
Justin D. Radolf
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.24.8004-8008.1991
Subject(s) - bacterial outer membrane , borrelia burgdorferi , biology , borrelia , immunogen , membrane protein , intracellular , membrane , microbiology and biotechnology , biophysics , biochemistry , escherichia coli , antibody , genetics , monoclonal antibody , gene
Borrelia burgdorferi B31 with and without outer membranes contained nearly identical amounts of outer surface proteins A and B. The majority of each immunogen also was localized intracellularly by immunocryoultramicrotomy. These results are inconsistent with the widely held belief that outer surface proteins A and B are exclusively outer membrane proteins.