Open AccessParticipation of ABF-1 protein in expression of the Saccharomyces cerevisiae CAR1 gene
Author(s) -
L. Z. Kovari,
Terrance G. Cooper
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.20.6332-6338.1991
Subject(s) - biology , saccharomyces cerevisiae , reporter gene , gene , microbiology and biotechnology , transcription (linguistics) , yy1 , dna , gatad2b , regulation of gene expression , transcriptional regulation , gene expression , dna binding protein , transcription factor , genetics , promoter , repressor , linguistics , philosophy
DNA fragments previously shown to be required for expression of the CAR1 (arginase) gene in Saccharomyces cerevisiae and to support transcriptional activation of a reporter gene in a heterologous expression vector were shown to bind purified regulatory protein ABF-1. Two ABF-1 sites were identified in the CAR1 upstream region, one to which ABF-1 protein bound with high affinity and a second to which it bound much less avidly. The higher-affinity ABF-1 binding site upstream of CAR1 was an effective competitor of the HMRE, ARS1 B domain, and COR2-GFI binding sequences for protein binding. Point mutations in the CAR1 high-affinity ABF-1 binding site resulted in a 12-fold loss of transcriptional activation of a reporter gene compared with the wild-type CAR1 DNA fragment. These data are consistent with the suggestion that ABF-1 protein is one of the transcription factors involved in expression of the CAR1 gene.