Open AccessCloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanism
Author(s) -
Michael J. Calcutt,
Eric Cundliffe
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.8.4710-4714.1990
Subject(s) - biology , lincosamides , streptomyces , streptomycetaceae , 23s ribosomal rna , genetics , microbiology and biotechnology , cloning (programming) , actinomycetales , antibiotic resistance , bacteria , gene , rna , ribosome , computer science , programming language
Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a single residue of N6-monomethyladenine in 23S rRNA at position 2058, thereby rendering the 50S ribosmal subunit resistant to the action of lincosamides.