Open AccessNucleotide sequence of the Pseudomonas aeruginosa phoB gene, the regulatory gene for the phosphate regulon
Author(s) -
Jamila Anba,
Michel Bidaud,
Michael L. Vasil,
Andrée Lazdunski
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.8.4685-4689.1990
Subject(s) - regulon , biology , nucleic acid sequence , escherichia coli , gene , open reading frame , peptide sequence , gene product , regulator gene , genetics , operon , pseudomonas aeruginosa , biochemistry , regulation of gene expression , bacteria , gene expression
The nucleotide sequence of Pseudomonas aeruginosa phoB was determined. The sequence data suggest that the PhoB polypeptide consists of 229 amino acid residues and has a predicted molecular weight of 25,708. In the regulatory region of the gene, a very well conserved phosphate box was found. The sequence data also predicted the presence of an open reading frame downstream of phoB, which could be phoR. The deduced amino acid sequence of phoB was significantly homologous to that of the Escherichia coli phoB gene product and to those of several known procaryotic transcriptional regulators such as PhoP, OmpR, VirG, Dye, NtrC, and AlgR.