Open AccessTranscriptional induction of purple membrane and gas vesicle synthesis in the archaebacterium Halobacterium halobium is blocked by a DNA gyrase inhibitor
Author(s) -
Chin-Fen Yang,
Shiladitya DasSarma
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.7.4118-4121.1990
Subject(s) - biology , dna gyrase , novobiocin , halobacterium , gene , transcription (linguistics) , gene expression , dna , bacillus subtilis , microbiology and biotechnology , halobacterium salinarum , biochemistry , escherichia coli , genetics , halophile , bacteria , archaea , linguistics , philosophy
We have investigated the expression of the bacteriorhodopsin gene (bop) and the gas vesicle protein gene (gvpA) in the extremely halophilic archaebacterium Halobacterium halobium, using primer-directed reverse transcription of RNA to quantify message levels. The level of gvpA gene transcript was found to increase about 5-fold from early to mid-logarithmic growth phase, while the level of bop gene transcript increased about 20-fold from mid-logarithmic to stationary phase. Transcriptional induction of both the gvpA and bop genes was significantly reduced by aeration and almost completely blocked by the DNA gyrase inhibitor novobiocin.