Open AccessVirulence genes promote conjugative transfer of the Ti plasmid between Agrobacterium strains
Author(s) -
Todd R. Steck,
Clarence I. Kado
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.4.2191-2193.1990
Subject(s) - acetosyringone , agrobacterium tumefaciens , plasmid , ti plasmid , biology , virulence , operon , t dna binary system , mutant , transformation (genetics) , microbiology and biotechnology , genetics , transfer dna , agrobacterium , gene , vector (molecular biology) , recombinant dna
Certain virulence region operons of the Agrobacterium tumefaciens Ti plasmid promoted conjugative Ti plasmid transfer. Mutations in the vir region of pTiC58 inhibited conjugative plasmid transfer between A. tumefaciens strains. Mutations in virA, virG, 5' virB, and virE had the greatest effect on plasmid transfer, and mutations in virC had no effect. Transfer inhibition in vir mutants occurred in the presence or absence of acetosyringone.