Open AccessIsolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene
Author(s) -
Rhonda D. Cardin,
A J Biel
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.4.2181-2183.1990
Subject(s) - biology , cosmid , rhodobacter , mutant , rpon , ecori , cloning (programming) , escherichia coli , genetics , gene , plasmid , microbiology and biotechnology , gene expression , promoter , computer science , programming language
We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.