Open AccessLysis protein S of phage lambda functions in Saccharomyces cerevisiae
Author(s) -
Jinnie M. Garrett,
Claudia Bruno,
Ry Young
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.12.7275-7277.1990
Subject(s) - biology , saccharomyces cerevisiae , escherichia coli , lysis , lambda phage , gene product , yeast , biochemistry , microbiology and biotechnology , cytoplasm , cell membrane , galactose , membrane , gene , gene expression , bacteriophage
The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.