Open AccessRecovery of DNA from the Acinetobacter calcoaceticus chromosome by gap repair
Author(s) -
Leslie A. Gregg-Jolly,
L N Ornston
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.10.6169-6172.1990
Subject(s) - acinetobacter calcoaceticus , biology , genetics , plasmid , circular bacterial chromosome , dna , gene , chromosome , transformation (genetics) , acinetobacter , microbiology and biotechnology , bacteria , dna replication
A strain of Acinetobacter calcoaceticus that demonstrates unusually high competence for natural transformation by linear DNA has proven valuable for analysis of genes and gene clusters associated with aromatic catabolism. The transformation system allowed gap repair to be used to recover mutant chromosomal DNA within recombinant plasmids. The sizes of the recovered fragments, 5 and 7 kilobase pairs in length, indicate that gap repair will be a useful procedure for isolation of wild-type and modified gene clusters from the A. calcoaceticus chromosome.