Open AccessBoth CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli
Author(s) -
Matthew P. Conley,
Audrey E Wolfe,
David F. Blair,
Howard C. Berg
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.9.5190-5193.1989
Subject(s) - chemotaxis , biology , flagellum , clockwise , escherichia coli , cytoplasm , microbiology and biotechnology , receptor , rotation (mathematics) , biochemistry , gene , geometry , mathematics
If cells of Escherichia coli deleted for genes that specify transducers and all known cytoplasmic chemotaxis proteins are reconstituted with CheA, CheW, and CheY, they spin their flagella alternately clockwise and counterclockwise. If the aspartate receptor also is present, clockwise rotation is suppressed upon addition of aspartate. If either CheA or CheW is absent, the fraction of time that the flagella spin clockwise is reduced and responses to aspartate do not occur.