Open AccessBacteriophage association of streptococcal pyrogenic exotoxin type C
Author(s) -
S C Goshorn,
Patrick M. Schlievert
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.6.3068-3073.1989
Subject(s) - biology , bacteriophage , dna , restriction enzyme , streptococcus pyogenes , gene , microbiology and biotechnology , restriction fragment length polymorphism , exotoxin , restriction fragment , restriction map , ecori , genetics , escherichia coli , bacteria , genotype , plasmid , staphylococcus aureus , toxin
A gene encoding streptococcal pyrogenic exotoxin type C (SPE C) was isolated from bacteriophage DNA derived from Streptococcus pyogenes CS112. The gene, designated speC2, was shown to reside near the phage attachment site of phage CS112. A restriction endonuclease map of the CS112 phage was generated, and the location and orientation of the speC2 gene were determined. Hybridization analyses of eight SPE C-producing strains revealed restriction fragment length polymorphism of the speC gene-containing DNA fragments and further showed that each speC was linked to a common CS112 phage-derived DNA fragment.