Open AccessLocalization of the replication origin of plasmid pE194
Author(s) -
Laurie A Dempsey,
David Dubnau
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.5.2866-2869.1989
Subject(s) - biology , origin of replication , plasmid , heterologous , replication (statistics) , genetics , origin recognition complex , dna replication , computational biology , dna , eukaryotic dna replication , virology , gene
The pE194 replication origin was localized to a 265-base-pair interval by analyzing the ability of purified pE194 restriction fragments to direct replication of heterologous plasmids. Replication was dependent upon RepF protein supplied in trans. The origin region contained a GC-rich dyad symmetry which may serve as the RepF target.