Open AccessRegulation of transcription of the Bacillus subtilis spoIIA locus
Author(s) -
Jiunn-Jong Wu,
M Howard,
Patrick J. Piggot
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.2.692-698.1989
Subject(s) - biology , sigma factor , transcription (linguistics) , promoter , genetics , start codon , primer extension , locus (genetics) , general transcription factor , rna polymerase , open reading frame , bacillus subtilis , transcription factor ii d , microbiology and biotechnology , gene , messenger rna , rna , gene expression , peptide sequence , bacteria , linguistics , philosophy
The start point of spoIIA transcription was defined by primer extension analysis with two separate primers. It was 27 bases upstream from the putative translation initiation codon of the first open reading frame in the spoIIA locus. A region extending at least 52 bases upstream from the transcription start site was necessary for transcription, as determined with integrative plasmids. Transcription of spoIIA was dependent on the spoOA, spoOB, and spoOF loci, but this dependency was partly overcome by increasing the number of copies of the spoIIA promoter region. Transcription of spoIIA was absolutely dependent on the spoOH locus, which codes for the RNA polymerase sigma factor sigma H. Regions approximately -35 and -10 upstream from the spoIIA transcription start site showed sequence homology with Bacillus subtilis sigma H promoters.