Open AccessPurification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA
Author(s) -
Yoshiyuki Kamio,
Yoshifumi Itoh,
Yoshiro Terawaki
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.9.4411-4414.1988
Subject(s) - biology , dna , plasmid , genetics , dna binding protein , binding site , gene , transcription factor
RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used.