Open AccessEvidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs
Author(s) -
M N Subbarao,
David Kennell
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.6.2860-2865.1988
Subject(s) - biology , lac repressor , lac operon , messenger rna , transcription (linguistics) , ribosome , nucleotide , translation (biology) , ribosomal binding site , genetics , microbiology and biotechnology , rna , gene , gene expression , linguistics , philosophy
S1 nuclease mapping revealed lacZ mRNA molecules whose 5' and 3' ends were internal to the transcription start and consistent with cleavages at pyrimidine-adenosine bonds 20 to 50 nucleotides apart. With the net 5'-to-3' direction known, lacZ mRNA is probably degraded by sequential cleavages of naked mRNA at vulnerable sites exposed by transit of the last translating ribosome.