Open AccessIsolation and characterization of the modification methylase M . SinI
Author(s) -
Christiaan Karreman,
A. de Waard
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.6.2533-2536.1988
Subject(s) - biology , plasmid , recombinant dna , escherichia coli , restriction enzyme , dna , microbiology and biotechnology , endonuclease , cleavage (geology) , enterobacteriaceae , nucleotide , nucleic acid sequence , biochemistry , enzyme , gene , paleontology , fracture (geology)
A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).