Open AccessImmunoelectron microscopic localization of small, acid-soluble spore proteins in sporulating cells of Bacillus subtilis
Author(s) -
Stephen C. Francesconi,
T J MacAlister,
Barbara Setlow,
Peter Setlow
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.12.5963-5967.1988
Subject(s) - immunoelectron microscopy , bacillus subtilis , biology , nucleoid , alpha (finance) , spore , beta (programming language) , microbiology and biotechnology , biochemistry , gene , bacteria , genetics , escherichia coli , antibody , programming language , medicine , construct validity , nursing , computer science , patient satisfaction
Small, acid-soluble spore proteins SASP-alpha, SASP-beta, and SASP-gamma as well as a SASP-beta-lacZ gene fusion product were found only within the forespore compartment of sporulating Bacillus subtilis cells by using immunoelectron microscopy. The alpha/beta-type SASP were associated almost exclusively with the forespore nucleoid, while SASP-gamma was somewhat excluded from the nucleoid. These different locations of alpha/beta-type and gamma-type small, acid-soluble spore proteins within the forespore are consistent with the different roles for these two types of proteins in spore resistance to UV light.