Open AccessAltered osmoregulation of ompF in integration host factor mutants of Escherichia coli
Author(s) -
Ping Tsui,
V. Helu,
Martin Freundlich
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.10.4950-4953.1988
Subject(s) - biology , mutant , porin , osmoregulation , osmotic concentration , escherichia coli , enterobacteriaceae , strain (injury) , gene , bacterial outer membrane , microbiology and biotechnology , genetics , biochemistry , ecology , salinity , anatomy
Osmoregulation of the porin protein OmpF was strongly altered in integration host factor (IHF) mutants. These mutants produced approximately 15-fold more OmpF than did the parent strain when grown in media of intermediate osmolarity. At high osmolarity IHF mutants continued to produce considerable amounts of OmpF, although this protein was undetectable in the parent grown under these conditions. Experiments with an ompF-lacZ chromosomal fusion strain suggested that these changes in osmoregulation in large part involve alterations in transcriptional activity of the ompF promoter. These results add to the growing list of genes whose expression is modified in IHF mutants.