Open AccessTn5-mediated transposition of plasmid DNA after transduction to Myxococcus xanthus
Author(s) -
J S Downard
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.10.4939-4941.1988
Subject(s) - myxococcus xanthus , biology , plasmid , transposition (logic) , transduction (biophysics) , dna , escherichia coli , genetics , microbiology and biotechnology , chromosome , circular bacterial chromosome , dna replication , gene , biochemistry , linguistics , philosophy , mutant
After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by intact Tn5 or IS50 sequences. These results suggest that Tn5-mediated transposition has occurred and provide a method for integrating plasmid DNA into the M. xanthus chromosome without the requirement for homologous recombination.