Open AccessEffects of release factor context at UAA codons in Escherichia coli
Author(s) -
Robert H. Martin,
Michael P. Weiner,
Jonathan Gallant
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.10.4714-4717.1988
Subject(s) - release factor , biology , transfer rna , escherichia coli , genetics , context (archaeology) , gene , stop codon , ribosome , nonsense mutation , nonsense , mutation , rna , missense mutation , paleontology
In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and the translational release factors RF1 and RF2. We have employed plasmids carrying the genes for RF1 and RF2 to measure release factor preference at UAA codons at 13 different sites in the lacI gene. We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites.